Article
A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity.
PloS one - 1 Jan 2012
Vos Michiel, Quince Christopher, Pijl Agata S, de Hollander Mattias, Kowalchuk George A
Abstract excerpt
BACKGROUND: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in an evolutionary framework. We compared the 1...
Topics
- DNA, Bacterial
- Genetic Markers
- Genetic Variation
- Phylogeny
- Proteobacteria
- RNA Polymerase II
- RNA, Ribosomal, 16S
- Sequence Analysis, DNA