Article
A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity.
PloS one - 1 Jan 2012
Vos Michiel, Quince Christopher, Pijl Agata S, de Hollander Mattias, Kowalchuk George A
Abstract excerpt
BACKGROUND: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in an evolutionary framework. We compared the 16S rRNA marker with the single-copy, protein-coding rpoB marker by amplifying and sequencing both from a single soil...
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