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Article

Capturing greater 16S rRNA gene sequence diversity within the domain Bacteria.

Applied and environmental microbiology - 1 Aug 2012

Winsley T, van Dorst J M, Brown M V, Ferrari B C

Abstract excerpt

A large proportion of "universal" 16S PCR primers lack sequence homology to many of the "candidate" divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in recovery of...

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Identifiers

PMID: 22685133
DOI: 10.1128/AEM.01299-12
PMCID: PMC3406127

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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