Article
Capturing greater 16S rRNA gene sequence diversity within the domain Bacteria.
Applied and environmental microbiology - 1 Aug 2012
Winsley T, van Dorst J M, Brown M V, Ferrari B C
Abstract excerpt
A large proportion of "universal" 16S PCR primers lack sequence homology to many of the "candidate" divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in recovery of...
Topics
- Bacteria
- DNA Primers
- DNA, Bacterial
- DNA, Ribosomal
- Genes, rRNA
- Genetic Variation
- Metagenomics
- Polymerase Chain Reaction
- RNA, Ribosomal, 16S
- Sequence Analysis, DNA
- Soil Microbiology