Article

Thousands of primer-free, high-quality, full-length SSU rRNA sequences from all domains of life

2016-08-22

Abstract excerpt

Ribosomal RNA (rRNA) genes are the consensus marker for determination of microbial diversity on the planet, invaluable in studies of evolution and, for the past decade, high-throughput sequencing of variable regions of ribosomal RNA genes has become the backbone of most microbial ecology studies. However, the underlying reference databases of full-length rRNA gene sequences are underpopulated, ecosystem skewed 1...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: ecbb4928-2553-5a97-b00d-7979cacd911e
DOI: 10.1101/070771

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Thousands of primer-free, high-quality, full-length SSU rRNA sequences from all domains of lifeDOI 10.1101/070771Read

Evaluating and Improving SSU rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean SurveysSemantic proximity Read

A novel long-amplicon <i>rpoB</i> primer pair for high resolution microbiome analysis at the species-levelSemantic proximity Read

The effect of primer choice and short read sequences on the outcome of 16S rRNA gene based diversity studies.Semantic proximity Read

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies.Semantic proximity Read

Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing.Semantic proximity Read

Towards a balanced view of the bacterial tree of life.Semantic proximity Read

Design of potentially universal SSU primers in myxomycetes using next-generation sequencing.Semantic proximity Read

Comparative analysis of full-length 16s ribosomal RNA genome sequencing in human fecal samples using primer sets with different degrees of degeneracySemantic proximity Read

Lessons and considerations for the creation of universal primers targeting non-conserved, horizontally mobile genesSemantic proximity Read

Mitag4taxa: Extracting SSU rRNA Illumina reads from metagenomes for taxonomic classificationSemantic proximity Read

Accurate Estimation of Fungal Diversity and Abundance through Improved Lineage-Specific Primers Optimized for Illumina Amplicon Sequencing.Semantic proximity Read

A universal set of primers to study animal associated microeukaryotic communitiesSemantic proximity Read