Article
Sequence analysis of two temperature-sensitive mutations in the alpha subunit gene (rpoA) of Escherichia coli RNA polymerase.
Nucleic acids research - 25 Oct 1990
Igarashi K, Fujita N, Ishihama A
Abstract excerpt
The rpoA gene of Escherichia coli encodes the alpha subunit of the DNA-dependent RNA polymerase. Two mutant alleles, rpoA101 and rpoA112, both of which produce RNA polymerase with altered thermostability and reduced fidelity of transcription in vitro (Ishihama et al. (1980) J. Mol. Biol. 137, 137...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- DNA-Directed RNA Polymerases
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes