Article
RNase HIII from Chlamydophila pneumoniae can efficiently cleave double-stranded DNA carrying a chimeric ribonucleotide in the presence of manganese.
Molecular microbiology - 1 Mar 2012
Lu Zheng, Liang Rubing, Liu Xipeng, Hou Jingli, Liu Jianhua
Abstract excerpt
Two ribonuclease Hs (RNase Hs) have been found in Chlamydophila pneumoniae, CpRNase HII and CpRNase HIII. This work is the first report that CpRNase HIII can efficiently cleave DNA-rN(1) -DNA/DNA (rN(1) , monoribonucleotide) in vitro in the presence of Mn(2+) , whereas the enzymatic activity of CpRNase HII on the same substrate was inhibited by Mn(2+) and dependent on Mg(2+) . However, the ability of both...
Topics
- Bacterial Proteins
- Chlamydophila pneumoniae
- DNA
- DNA, Bacterial
- Manganese
- Mutation
- Ribonucleases
- Ribonucleotides