Article
Gene cloning and characterization of recombinant RNase HII from a hyperthermophilic archaeon.
Journal of bacteriology - 1 Dec 1998
Haruki M, Hayashi K, Kochi T, Muroya A, Koga Y, Morikawa M, Imanaka T, Kanaya S
Abstract excerpt
We have cloned the gene encoding RNase HII (RNase HIIPk) from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 by screening of a library for clones that suppressed the temperature-sensitive growth phenotype of an rnh mutant strain of Escherichia coli. This gene was expressed in an rnh...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Primers
- DNA, Archaeal
- Enzyme Stability
- Escherichia coli
- Genes, Archaeal
- Molecular Sequence Data
- Mutation
- Pyrococcus
- RNA, Archaeal
- RNA, Ribosomal, 16S
- Recombinant Proteins
- Ribonuclease H
- Sequence Homology, Amino Acid
- Substrate Specificity
- Temperature