Article
Effect of the termini of RNase Hs from Chlamydophila pneumoniae on enzymatic biochemical characterization.
Acta biochimica et biophysica Sinica - 1 Oct 2012
Hou Jingli, Lu Zheng, Guo Xingliang, Liu Jianhua
Abstract excerpt
A difference between prokaryotic RNase HII and HIII, which both belong to type 2 RNase H, is a long N-terminal extension of HIII; however, the main-fold structures of HII and HIII known as RNase H-fold are similar. To further understand the structure-function relationship of RNase HII and RNase HIII, biochemical analyses were carried out using N-terminal truncations of RNase HIII (IIIN56(Δ), IIIN81(Δ), and...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- Chlamydophila pneumoniae
- Circular Dichroism
- DNA
- Electrophoresis, Polyacrylamide Gel
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutation