Article
Involvement of Ser94 in RNase HIII from Chlamydophila pneumoniae in the recognition of a single ribonucleotide misincorporated into double-stranded DNA.
Biochimica et biophysica acta - 1 Jul 2012
Lu Zheng, Hou Jingli, Wang You, Liu Jianhua
Abstract excerpt
We recently provided the first report that RNase HIII can cleave a DNA-rN(1)-DNA/DNA substrate (rN(1), one ribonucleotide) in vitro. In the present study, mutagenesis analyses and molecular dynamics (MD) simulations were performed on RNase HIII from Chlamydophila pneumoniae AR39 (CpRNase HIII). Our results elucidate the mechanism of ribonucleotide recognition employed by CpRNase HIII, indicating that the...
Topics
- Amino Acid Motifs
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- Chlamydophila pneumoniae
- DNA, Bacterial
- Hydrogen Bonding
- Molecular Dynamics Simulation
- Molecular Sequence Data
- Mutation
- Protein Structure, Tertiary
- RNA, Bacterial
- Recombinant Proteins