Article
MnlI--The member of H-N-H subtype of Type IIS restriction endonucleases.
Biochimica et biophysica acta - 10 Aug 2005
Kriukiene Edita, Lubiene Judita, Lagunavicius Arunas, Lubys Arvydas
Abstract excerpt
The Type IIS restriction endonuclease MnlI recognizes the non-palindromic nucleotide sequence 5'-CCTC(N)7/6 downward arrow and cleaves DNA strands as indicated by the arrow. The genes encoding MnlI restriction-modification system were cloned and sequenced. It comprises N6-methyladenine and C5-methylcytosine methyltransferases and the restriction endonuclease. Biochemical studies revealed that MnlI restriction...
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