New modules for PCR‐based gene targeting in <i>Candida albicans</i>: rapid and efficient gene targeting using 100 bp of flanking homology region

Abstract excerpt

The use of PCR-based techniques for directed gene alterations has become a standard tool in Saccharomyces cerevisiae. In our efforts to increase the speed of functional analysis of Candida albicans genes, we constructed a modular system of plasmid vectors and successfully applied PCR-amplified functional analysis (FA)-cassettes in the transformation of C. albicans. These cassettes facilitate: (a) gene...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Antifungal resistance and susceptibility
• Share to Fungal Infections and Studies
• Share to Peptidase Inhibition and Analysis