Article
Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration.
Molecular & general genetics : MGG - 24 Apr 1996
Brown D H, Slobodkin I V, Kumamoto C A
Abstract excerpt
To allow the regulated expression of cloned genes in Candida albicans, a plasmid was constructed using the inducible promoter of the C. Albicans MAL2 gene. To demonstrate that the MAL2 promoter could regulate cloned genes placed under its control, a fusion construct was made with the coding sequence of the C. albicans URA3 gene. This plasmid was introduced into a Ura- strain of C. albicans using the process of...
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