Article
The vital role of polymerase ζ and REV1 in mutagenic, but not correct, DNA synthesis across benzo[a]pyrene-dG and recruitment of polymerase ζ by REV1 to replication-stalled site.
The Journal of biological chemistry - 16 Mar 2012
Hashimoto Keiji, Cho Youngjin, Yang In-Young, Akagi Jun-ichi, Ohashi Eiji, Tateishi Satoshi, de Wind Niels, Hanaoka Fumio, Ohmori Haruo, Moriya Masaaki
Abstract excerpt
The DNA synthesis across DNA lesions, termed translesion synthesis (TLS), is a complex process influenced by various factors. To investigate this process in mammalian cells, we examined TLS across a benzo[a]pyrene dihydrodiol epoxide-derived dG adduct (BPDE-dG) using a plasmid bearing a single BPDE-dG and genetically engineered mouse embryonic fibroblasts (MEFs). In wild-type MEFs, TLS was extremely miscoding...
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