Article
Characterization of a temperature-sensitive mutant in the RNA polymerase PB2 subunit gene of influenza A/WSN/33 virus.
Archives of virology - 1 Jan 1990
Yamanaka K, Ogasawara N, Ueda M, Yoshikawa H, Ishihama A, Nagata K
Abstract excerpt
The temperature-sensitive mutant ts-1 of influenza virus A/WSN/33 carries mutations in the gene encoding RNA polymerase PB2 subunit. Effect of temperature on various steps of viral RNA synthesis was examined using disrupted virions of ts-1 mutant. The initiation of RNA synthesis with dinucleotide ApG primer was not affected by elevated temperature, whereas that with primer RNA containing 5'-terminal cap-1...
Topics
- Amino Acid Sequence
- Base Sequence
- Cells, Cultured
- Cloning, Molecular
- DNA Mutational Analysis
- DNA, Viral
- DNA-Directed RNA Polymerases
- Genes, Viral
- Influenza A virus
- Molecular Sequence Data
- Mutation