Article
Mutational analysis identifies functional domains in the influenza A virus PB2 polymerase subunit.
Journal of virology - 1 Mar 1996
Perales B, de la Luna S, Palacios I, Ortín J
Abstract excerpt
A collection of influenza virus PB2 mutant genes was prepared, including N-terminal deletions, C-terminal deletions, and single-amino-acid insertions. These mutant genes, driven by a T7 promoter, were expressed by transfection into COS-1 cells infected with a vaccinia virus encoding T7 RNA polyme...
Topics
- Base Sequence
- Binding Sites
- Cell Line
- DNA, Viral
- Humans
- Influenza A virus
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- RNA Caps
- RNA-Dependent RNA Polymerase
- Repressor Proteins
- Sequence Deletion
- Transcription, Genetic
- Viral Proteins