Two mutations in the C-terminal domain of influenza virus RNA polymerase PB2 enhance transcription by enhancing cap-1 RNA binding activity.

doi:10.1016/j.bbagrm.2011.11.006

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Article

Two mutations in the C-terminal domain of influenza virus RNA polymerase PB2 enhance transcription by enhancing cap-1 RNA binding activity.

Biochimica et biophysica acta - 1 Jan 2012

Zhang Shijian, Wang Qiang, Wang Jinlan, Mizumoto Kiyohisa, Toyoda Tetsuya

Abstract excerpt

Influenza virus RNA polymerase (RdRp) PB2 is the cap-1 binding subunit and determines host range and pathogenicity. The mutant human influenza virus RdRp containing PB2 D701N and D701N/S714R demonstrated enhanced replicon activity in mammalian cells. We investigated the influence of these mutations on RdRp activity. Cap-1-dependent transcription activities of D701N/S714R, D701N, and S714R were 348.1±6.2%,...

Topics

Gene Expression Regulation, Viral
HEK293 Cells
Humans
Influenza A virus
Influenza, Human
Mutation
Nucleotidyltransferases
Protein Structure, Tertiary
RNA Cap-Binding Proteins
RNA Caps
RNA-Dependent RNA Polymerase
Transcription, Genetic
Viral Proteins

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Identifiers

PMID: 22146492
DOI: 10.1016/j.bbagrm.2011.11.006

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