Article
Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism.
Molecular and cellular probes - 1 Jan 2000
Gromadzka Beata, Smietanka Krzysztof, Dragun Jakub, Minta Zenon, Gora-Sochacka Anna, Szewczyk Boguslaw
Abstract excerpt
We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run using these primers, products were denatured and single-strand DNA fragments were subjected to...
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