Article
Use of illumina deep sequencing technology to differentiate hepatitis C virus variants.
Journal of clinical microbiology - 1 Mar 2012
Ninomiya Masashi, Ueno Yoshiyuki, Funayama Ryo, Nagashima Takeshi, Nishida Yuichiro, Kondo Yasuteru, Inoue Jun, Kakazu Eiji, Kimura Osamu, Nakayama Keiko, Shimosegawa Tooru
Abstract excerpt
Hepatitis C virus (HCV) is a positive-strand enveloped RNA virus that shows diverse viral populations even in one individual. Though Sanger sequencing has been used to determine viral sequences, deep sequencing technologies are much faster and can perform large-scale sequencing. We demonstrate the successful use of Illumina deep sequencing technology and subsequent analyses to determine the genetic variants and...
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