Article
Accurate genotyping of hepatitis C virus through nucleotide sequencing and identification of new HCV subtypes in China population.
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases - 1 Sept 2015
Tong Y-Q, Liu B, Liu H, Zheng H-Y, Gu J, Liu H, Song E J, Song C, Li Y
Abstract excerpt
Nucleotide sequencing of the phylogenetically informative region of NS5B remains the gold standard for hepatitis C virus (HCV) genotyping. Here we developed a new methodology for sequencing new NS5B regions to increase the accuracy and sensitivity of HCV genotyping and subtyping. The eight new primers were identified by scanning the full-length NS5B regions from 1127 HCV genomic sequences found in HCV databases....
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