Article
Engineering upper hinge improves stability and effector function of a human IgG1.
The Journal of biological chemistry - 17 Feb 2012
Yan Boxu, Boyd Daniel, Kaschak Timothy, Tsukuda Joni, Shen Amy, Lin Yuwen, Chung Shan, Gupta Priyanka, Kamath Amrita, Wong Anne, Vernes Jean-Michel, Meng Gloria Y, Totpal Klara, Schaefer Gabriele, Jiang Guoying, Nogal Bartek, Emery Craig, Vanderlaan Martin, Carter Paul, Harris Reed, Amanullah Ashraf
Abstract excerpt
Upper hinge is vulnerable to radical attacks that result in breakage of the heavy-light chain linkage and cleavage of the hinge of an IgG1. To further explore mechanisms responsible for the radical induced hinge degradation, nine mutants were designed to determine the roles that the upper hinge Asp and His play in the radical reactions. The observation that none of these substitutions could inhibit the breakage...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.