Article
Modulation of the effector functions of a human IgG1 through engineering of its hinge region.
Journal of immunology (Baltimore, Md. : 1950) - 15 Jul 2006
Dall'Acqua William F, Cook Kimberly E, Damschroder Melissa M, Woods Robert M, Wu Herren
Abstract excerpt
We report here the engineering of a humanized anti-human EphA2 mAb (mAb 12G3H11) in an effort to explore the relationship between the hinge of a human IgG1 and its effector functions. mAb 12G3H11, used here as a model, is directed against the human receptor tyrosine kinase EphA2, which is an actively investigated target for cancer therapy due to its up-regulation in many cancer cells. Various rational...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Antibodies, Monoclonal
- Antibody-Dependent Cell Cytotoxicity
- Binding Sites, Antibody
- Cell Line, Tumor
- Chromatography, Gel
- Complement C1q
- Complement Fixation Tests
- Cysteine
- Cytotoxicity Tests, Immunologic
- Genetic Variation
- Humans