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Article

A slow but steady nanoLuc: R162A mutation results in a decreased, but stable, nanoLuc activity.

International journal of biological macromolecules - 1 Jun 2024

Ahmed Wesam S, Geethakumari Anupriya M, Sultana Asfia, Fatima Asma, Philip Angelin M, Uddin S M Nasir, Biswas Kabir H

Abstract excerpt

NanoLuc (NLuc) luciferase has found extensive application in designing a range of biological assays, including gene expression analysis, protein-protein interaction, and protein conformational changes due to its enhanced brightness and small size. However, questions related to its mechanism of interaction with the substrate, furimazine, as well as bioluminescence activity remain elusive. Here, we combined...

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Identifiers

PMID: 38692549
DOI: 10.1016/j.ijbiomac.2024.131864

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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