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Rapid and reliable genotyping of HLA-B*27 in the Chinese Han population using a duplex real-time TaqMan PCR assay.

Clinical biochemistry - 1 Jan 2012

Fan Wen, Huang Lu, Zhou Zhiming, Zeng Xiaoqian, Li Guining, Deo Pankaj, Hu Lihua, Li Yirong

Abstract excerpt

OBJECTIVES: To develop a duplex real-time TaqMan PCR assay for genotyping HLA-B*27 in the Chinese Han population. DESIGN AND METHODS: A standard curve was constituted to deduce amplification efficiency, dynamic range and detection limit of the duplex real-time TaqMan PCR assay, whereas PCR-SBT (PCR with sequence-based typing) was used to evaluate the accuracy of the assay. RESULTS: A linear standard curve for...

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PMID: 22138519
DOI: 10.1016/j.clinbiochem.2011.11.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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