Article
Sterol-mediated regulation of mevalonic acid synthesis. Accumulation of 4-carboxysterols as the predominant sterols synthesized in a Chinese hamster ovary cell cholesterol auxotroph (mutant 215).
The Journal of biological chemistry - 5 Oct 1990
Plemenitas A, Havel C M, Watson J A
Abstract excerpt
Chinese hamster ovary-215 (CHO-215) mutant cells are auxotrophic for cholesterol. Berry and Chang (Berry, D. J., and Chang, T. Y. (1982) Biochemistry 21, 573-580) suggested that the metabolic lesion was at the level of 4-methyl sterol oxidation. However, the observed cellular accumulation of lanosterol was not consistent with a defect at this metabolic site. With the use of a novel Silica Sep Pak sterol...
Topics
- Animals
- Cell Line
- Cricetinae
- Cricetulus
- Female
- Homeostasis
- Hydroxymethylglutaryl CoA Reductases
- Mass Spectrometry
- Mevalonic Acid
- Mutation
- Ovary