Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Secretion in yeast of mutant human lysozymes with and without glutathione bound to cysteine 95.

The Journal of biological chemistry - 5 Oct 1990

Taniyama Y, Seko C, Kikuchi M

Abstract excerpt

A mutant human lysozyme C77A, in which Cys-77 is replaced with Ala, was secreted by Saccharomyces cerevisiae as two proteins (C77A-a and C77A-b) with different specific activities. A peptide fragment from Val93 to Ala108 was obtained from C77A-a by pepsin digestion, and examined by fast atom bombardment mass spectrometry and amino acid analysis. The results showed that glutathione was attached to the thiol group...

Topics

Amino Acid Sequence
Chromatography, High Pressure Liquid
Cysteine
Disulfides
Humans
Molecular Sequence Data
Muramidase
Mutation
Pepsin A
Peptide Fragments
Peptide Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Recombinant Proteins

Saccharomyces cerevisiae

Spectrometry, Mass, Fast Atom Bombardment

Source

Identifiers

PMID: 2211592

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2211592-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Secretion in yeast of mutant human lysozymes with and without glutathione bound to cysteine 95.PMID 2211592Read ↗

Folding mechanism of mutant human lysozyme C77/95A with increased secretion efficiency in yeast.Semantic proximity Read ↗

Occurrence of S-(1,2-dicarboxyethyl)-cysteine at position 77 in mutant human lysozyme secreted by Saccharomyces cerevisiae.Semantic proximity Read ↗

The crystal structure of a mutant human lysozyme C77/95A with increased secretion efficiency in yeast.Semantic proximity Read ↗

Accelerated secretion of human lysozyme with a disulfide bond mutation.Semantic proximity Read ↗

Evidence for difference in the roles of two cysteine residues involved in disulfide bond formation in the folding of human lysozyme.Semantic proximity Read ↗

Indication of possible post-translational formation of disulphide bonds in the beta-sheet domain of human lysozyme.Semantic proximity Read ↗

Stabilization and enhanced enzymatic activities of a mutant human lysozyme C77/95A with a cavity space by amino acid substitution.Semantic proximity Read ↗

Effect of chicken lysozyme signal peptide alterations on secretion of human lysozyme in Saccharomyces cerevisiae.Semantic proximity Read ↗

The relationship between disulphide bond formation, processing and secretion of lipo-beta-lactamase in yeast.Semantic proximity Read ↗

The specificity in vivo of two distinct methionine aminopeptidases in Saccharomyces cerevisiae.Semantic proximity Read ↗

Effect of N-terminal mutation of human lysozyme on enzymatic activity.Semantic proximity Read ↗

Crystal structure of a mutant human lysozyme with a substituted disulfide bond.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.