Article
The relationship between disulphide bond formation, processing and secretion of lipo-beta-lactamase in yeast.
Molecular microbiology - 1 Jan 1992
Shani O, Pines O
Abstract excerpt
The hybrid prokaryotic lipo-beta-lactamase mature and precursor proteins spontaneously form an intramolecular disulphide bond when oxidized in vitro. When expressed in Saccharomyces cerevisiae (in vivo) the lipo-beta-lactamase precursor is in a reduced form whereas the majority of the mature prot...
Topics
- Amino Acid Sequence
- Base Sequence
- Disulfides
- Electrophoresis, Polyacrylamide Gel
- Endoplasmic Reticulum
- Enzyme Precursors
- Molecular Sequence Data
- Mutation
- Oxidation-Reduction
- Precipitin Tests
- Protein Conformation
- Protein Processing, Post-Translational