Article
Occurrence of S-(1,2-dicarboxyethyl)-cysteine at position 77 in mutant human lysozyme secreted by Saccharomyces cerevisiae.
European journal of biochemistry - 26 Jan 1990
Kikuchi M, Taniyama Y, Kanaya S, Takao T, Shimonishi Y
Abstract excerpt
A mutant human lysozyme P110, in which Val110 was replaced with Pro, was secreted by Saccharomyces cerevisiae; modification of the cysteine residue at position 77 was found in a purified mutant protein (P110-B) upon primary structure analysis. A peptide fragment containing 15 amino acid residues...
Topics
- Amino Acid Sequence
- Amino Acids
- Binding Sites
- Chromatography, High Pressure Liquid
- Cysteine
- Humans
- Hydrolysis
- Mass Spectrometry
- Molecular Sequence Data
- Muramidase
- Mutation
- Peptide Fragments
- Peptide Mapping
- Plasmids
- Protein Engineering
- Recombinant Proteins
- Saccharomyces cerevisiae