Folding mechanism of mutant human lysozyme C77/95A with increased secretion efficiency in yeast.

Article

Folding mechanism of mutant human lysozyme C77/95A with increased secretion efficiency in yeast.

The Journal of biological chemistry - 5 Mar 1992

Taniyama Y, Ogasahara K, Yutani K, Kikuchi M

Abstract excerpt

A mutant human lysozyme C77/95A, in which Cys77 and Cys95 are replaced with alanine, has been characterized by 8-fold greater secretion in yeast (Taniyama, Y., Yamamoto, Y., Nakao, M., Kikuchi, M., and Ikehara, M. (1988) Biochem. Biophys. Res. Commun. 152, 962-967) and almost the same three-dimen...

Topics

Circular Dichroism
Disulfides
Fluorescence Polarization
Humans
Kinetics
Muramidase
Mutation
Protein Conformation
Protein Denaturation
Saccharomyces cerevisiae
X-Ray Diffraction

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.