Article
Change of a single amino acid in the leader peptide of a staphylococcal beta-lactamase prevents the appearance of the enzyme in the medium.
FEMS microbiology letters - 1 Jun 1990
East A K, Curnock S P, Dyke K G
Abstract excerpt
A plasmid encoded beta-lactamase gene from Staphylococcus aureus (strain 3804) was cloned and sequenced. The nucleotide sequence is very similar to those obtained for other such beta-lactamase genes. The beta-lactamase has an N-terminal region characteristic of an exported protein and assay of activity shows that the enzyme is found extracellularly in S. aureus. A residue in the N-terminal region near to the...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Protein Sorting Signals
- Restriction Mapping
- Sequence Homology, Nucleic Acid