Article
Mutation of serine residue 318 in the class C beta-lactamase of Enterobacter cloacae 908R.
FEMS microbiology letters - 1 Apr 1992
Jacobs C, Dubus A, Monnaie D, Normark S, Frère J M
Abstract excerpt
The involvement of the serine residue 318 in the specificity of a class C beta-lactamase was investigated. Multiple site-directed mutants at this position were generated using a polymerase chain reaction technique. These mutants were then probed for their activity towards various beta-lactam comp...
Topics
- Base Sequence
- Drug Resistance, Microbial
- Enterobacter cloacae
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Denaturation
- Substrate Specificity
- beta-Lactamases