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Article

A high-throughput microfluidic method for generating and characterizing transcription factor mutant libraries.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Geertz Marcel, Rockel Sylvie, Maerkl Sebastian J

Abstract excerpt

Characterizing libraries of mutant proteins is a challenging task, but can lead to detailed functional insights on a specific protein, and general insights for families of proteins such as transcription factors. Challenges in mutant protein screening consist in synthesizing the necessary expression-ready DNA constructs and transforming them into a suitable host for protein expression. Protein purification and...

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PMID: 22083738
DOI: 10.1007/978-1-61779-412-4_6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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