Article
Developing a Nanopore Sequencing Workflow for Protein Engineering Applications.
ACS synthetic biology - 21 Jul 2023
Tan Daniel Zhi Jun, Fung Vincent, Sun Tao, Tian Kaiyuan, Li Zhi, Zhou Kang
Abstract excerpt
Sequencing plays a critical role in protein engineering, where the genetic information encoding for a desired mutation can be identified. We evaluated the performance of two commercially available NGS technologies (Illumina NGS and nanopore sequencing) on the available mutant libraries that were either previously constructed for other protein engineering projects or constructed in-house for this study. The...
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