Article
Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis.
Proceedings of the National Academy of Sciences of the United States of America - 30 Sept 1997
Pelicic V, Jackson M, Reyrat J M, Jacobs W R, Gicquel B, Guilhot C
Abstract excerpt
A better understanding of Mycobacterium tuberculosis virulence mechanisms is highly dependent on the design of efficient mutagenesis systems. A system enabling the positive selection of insertional mutants having lost the delivery vector was developed. It uses ts-sacB vectors, which combine the c...
Topics
- Alleles
- BCG Vaccine
- Bacterial Proteins
- Base Sequence
- DNA Transposable Elements
- DNA, Bacterial
- Genes, Bacterial
- Mutagenesis, Insertional
- Mycobacterium bovis
- Mycobacterium tuberculosis
- Peptide Synthases