Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Engineering the genome of Thermus thermophilus using a counterselectable marker.

Journal of bacteriology - 1 Mar 2015

Carr Jennifer F, Danziger Michael E, Huang Athena L, Dahlberg Albert E, Gregory Steven T

Abstract excerpt

UNLABELLED: Thermus thermophilus is an extremely thermophilic bacterium that is widely used as a model thermophile, in large part due to its amenability to genetic manipulation. Here we describe a system for the introduction of genomic point mutations or deletions using a counterselectable marker consisting of a conditionally lethal mutant allele of pheS encoding the phenylalanyl-tRNA synthetase α-subunit. Mutant...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to beta-Galactosidase
Share to Gene Expression Regulation, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Expression Regulation, Enzymologic

Share to Genetic Engineering

Share to Genome, Bacterial

Share to RNA, Bacterial

Share to RNA, Ribosomal, 16S

Share to Thermus thermophilus

Source

Open in PubMed Open full text

Identifiers

PMID: 25605305
DOI: 10.1128/JB.02384-14
PMCID: PMC4336342

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Engineering the genome of Thermus thermophilus using a counterselectable marker.PMID 25605305Read ↗

Counterselection employing mutated pheS for markerless genetic deletion in Bacteroides species.Semantic proximity Read ↗

Highly Effective Markerless Genetic Manipulation of Streptococcus suis Using a Mutated PheS-Based Counterselectable MarkerSemantic proximity Read ↗

Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons of Escherichia coli.Semantic proximity Read ↗

Development of SacB-based Counterselection for Efficient Allelic Exchange in <i>Fusobacterium nucleatum</i>Semantic proximity Read ↗

Directly fishing out subtle mutations in genomic DNA with histidine-tagged Thermus thermophilus MutS.Semantic proximity Read ↗

Multiple genetic tools for editing the genome of Riemerella anatipestifer using a counterselectable marker.Semantic proximity Read ↗

Single base alterations upstream of the E. coli 16S rRNA coding region result in temperature-sensitive 16S rRNA expression.Semantic proximity Read ↗

Adaptation of Prokaryotic Toxins for Negative Selection and Cloning-Independent Markerless Mutagenesis in Streptococcus Species.Semantic proximity Read ↗

Generation of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated pheS for Counterselection.Semantic proximity Read ↗

CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida.Semantic proximity Read ↗

Development of a markerless genetic exchange method for Methanosarcina acetivorans C2A and its use in construction of new genetic tools for methanogenic archaea.Semantic proximity Read ↗

Genetic Tools for Allelic Replacement in Burkholderia SpeciesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.