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Article

Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe.

Nature protocols - 1 Jan 2007

Gregan Juraj, Zhang Chao, Rumpf Cornelia, Cipak Lubos, Li Zhang, Uluocak Pelin, Nasmyth Kim, Shokat Kevan M

Abstract excerpt

Reversible protein phosphorylation is a major regulatory mechanism in a cell. A chemical-genetic strategy to conditionally inactivate protein kinases has been developed recently. Mutating a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors. The inhibitor ca...

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Identifiers

PMID: 18007635
DOI: 10.1038/nprot.2007.447
PMCID: PMC2957860

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-18007635-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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