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Directed evolution improves the fibrinolytic activity of nattokinase from Bacillus natto.

FEMS microbiology letters - 1 Dec 2011

Yongjun Cai, Wei Bao, Shujun Jiang, Meizhi Weng, Yan Jia, Yan Yin, Zhongliang Zheng, Goulin Zou

Abstract excerpt

Nattokinase (subtilisin NAT, NK) is a relatively effective microbial fibrinolytic enzyme that has been identified and characterized from Bacillus natto. In the current report, DNA family shuffling was used to improve the fibrinolytic activity of nattokinase. Three homologous genes from B. natto AS 1.107, Bacillus amyloliquefaciens CICC 20164 and Bacillus licheniformis CICC 10092 were shuffled to generate a mutant...

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PMID: 22029857
DOI: 10.1111/j.1574-6968.2011.02423.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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