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Construction of highly active and stable recombinant nattokinase by engineered bacteria and computational design.

Archives of biochemistry and biophysics - 1 Oct 2024

Wang Lianxin, Meng Jinhui, Yu Xiaomiao, Wang Jie, Zhang Yuying, Zhang Man, Zhang Yuxi, Wang Hengyi, Feng Huawei, Tian Qifeng, Zhang Li, Liu Hongsheng

Abstract excerpt

Nattokinase (NK) is an enzyme that has been recognized as a new potential thrombolytic drug due to its strong thrombolytic activity. However, it is difficult to maintain the enzyme activity of NK during high temperature environment of industrial production. In this study, we constructed six NK mutants with potential for higher thermostability using a rational protein engineering strategy integrating free...

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PMID: 39154817
DOI: 10.1016/j.abb.2024.110126

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