Article
Potent fibrinolytic enzyme from a mutant of Bacillus subtilis IMR-NK1.
Journal of agricultural and food chemistry - 1 Aug 2000
Chang C T, Fan M H, Kuo F C, Sung H Y
Abstract excerpt
A mutant of Bacillus subtilis IMR-NK1, which is used for the production of domestic "natto" in Taiwan, produced high fibrinolytic enzyme activity by solid-state fermentation using wheat bran as medium. In addition, a strong fibrinolytic enzyme was purified from the cultivation media. The purified enzyme was almost homogeneous, as examined by SDS-PAGE and capillary electrophoresis. The enzyme had an optimal pH of...
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