Article
Preparation of an activated rhodopsin/transducin complex using a constitutively active mutant of rhodopsin.
Biochemistry - 29 Nov 2011
Xie Guifu, D'Antona Aaron M, Edwards Patricia C, Fransen Maikel, Standfuss Jorg, Schertler Gebhard F X, Oprian Daniel D
Abstract excerpt
The interaction of rhodopsin and transducin has been the focus of study for more than 30 years, but only recently have efforts to purify an activated complex in detergent solution materialized. These efforts have used native rhodopsin isolated from bovine retina and employed either sucrose density gradient centrifugation or size exclusion chromatography to purify the complex. While there is general agreement on...
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