Article
Rhodopsin mutants that bind but fail to activate transducin.
Science (New York, N.Y.) - 5 Oct 1990
Franke R R, König B, Sakmar T P, Khorana H G, Hofmann K P
Abstract excerpt
Rhodopsin is a member of a family of receptors that contain seven transmembrane helices and are coupled to G proteins. The nature of the interactions between rhodopsin mutants and the G protein, transduction (Gt), was investigated by flash photolysis in order to monitor directly Gt binding and dissociation. Three mutant opsins with alterations in their cytoplasmic loops bound 11-cis-retinal to yield pigments with...
Topics
- Amino Acid Sequence
- Animals
- Cell Line
- Cell Membrane
- Chromosome Deletion
- Micelles
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Photolysis
- Protein Binding
- Protein Conformation