Article
Constitutively active rhodopsin mutants causing night blindness are effectively phosphorylated by GRKs but differ in arrestin-1 binding.
Cellular signalling - 1 Nov 2013
Vishnivetskiy Sergey A, Ostermaier Martin K, Singhal Ankita, Panneels Valerie, Homan Kristoff T, Glukhova Alisa, Sligar Stephen G, Tesmer John J G, Schertler Gebhard F X, Standfuss Joerg, Gurevich Vsevolod V
Abstract excerpt
The effects of activating mutations associated with night blindness on the stoichiometry of rhodopsin interactions with G protein-coupled receptor kinase 1 (GRK1) and arrestin-1 have not been reported. Here we show that the monomeric form of WT rhodopsin and its constitutively active mutants M257Y, G90D, and T94I, reconstituted into HDL particles are effectively phosphorylated by GRK1, as well as two more...
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