Article
Differential high-resolution melting analysis for the detection of K-ras codons 12 and 13 mutations in pancreatic cancer.
Pancreas - 1 Nov 2011
Zhang Chunyan, Guo Wei, Wu Jiong, Song Binbin, Zhang Chen, Dai Qian, Pan Baishen, Ji Yuan, Guo Jinghui
Abstract excerpt
OBJECTIVE: The aim of this study was to establish a differential high-resolution melting analysis for the detection of K-ras codons 12 and 13 mutations in pancreatic cancer tissue. METHODS: We tested the sensitivity of this genotyping approach in cell lines with known K-ras mutations using 163- and 59-base pair (bp) amplicons spanning codons 12 and 13. We then screened 50 pancreatic cancer tissues, which were...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.