Article
Efficient and accurate KRAS genotyping using digital PCR combined with melting curve analysis for ctDNA from pancreatic cancer patients.
Scientific reports - 21 Feb 2023
Tanaka Junko, Nakagawa Tatsuo, Harada Kunio, Morizane Chigusa, Tanaka Hidenori, Shiba Satoshi, Ohba Akihiro, Hijioka Susumu, Takai Erina, Yachida Shinichi, Kamura Yoshio, Ishida Takeshi, Yokoi Takahide, Uematsu Chihiro
Abstract excerpt
A highly sensitive and highly multiplexed quantification technique for nucleic acids is necessary to predict and evaluate cancer treatment by liquid biopsy. Digital PCR (dPCR) is a highly sensitive quantification technique, but conventional dPCR discriminates multiple targets by the color of the fluorescent dye of the probe, which limits multiplexing beyond the number of colors of fluorescent dyes. We previously...
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