Article
Comparison of two different screening methods for the KRAS mutation in colorectal cancer.
Clinical laboratory - 1 Jan 2010
Er Tze-Kiong, Chang Ya-Sian, Yeh Kun-Tu, Chang Tien-Jye, Chang Jan-Gowth
Abstract excerpt
BACKGROUND: To compare high resolution melting (HRM) and primer extension (PE) for mutation screening of KRAS codons 12 and 13. METHODS: DNA samples were isolated from 60 colorectal cancer (CRC) patients. HRM and primer extension analyses were used for mutation screening of KRAS codons 12 and 13. RESULTS: Both methods can detect KRAS mutations in a DNA mixture containing as little as 5% mutant DNA. The concordant...
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