Article
A transformation system for the biocontrol yeast, Candida oleophila, based on hygromycin B resistance.
Current genetics - 1 Dec 2001
Yehuda H, Droby S, Wisniewski M, Goldway M
Abstract excerpt
Lithium acetate transformation and electroporation were applied to the biocontrol yeast, Candida oleophila. The hygromycin B resistance gene, flanked by the phosphoglycerate kinase promoter and terminator of Candida tropicalis, served as the genetic selection marker. The transformation efficiency of electroporation was almost 400 times more efficient than that of the lithium acetate method. While incorporation of...
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