Article
Isolation of Candida tropicalis auxotrophic mutants.
Applied and environmental microbiology - 1 Aug 1990
Gleeson M A, Haas L O, Cregg J M
Abstract excerpt
An enrichment scheme using nystatin was designed for the isolation of auxotrophic mutants from the diploid-alkane-utilizing yeast Candida tropicalis. A collection of 194 auxotrophs representing 7 phenotypes was isolated. One class of mutants was identified as having a defect in histidinol dehydrogenase activity and a second class of mutants was identified as having a defect in orotidine monophosphate...
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