Article
Nucleophosmin interacts with FOXM1 and modulates the level and localization of FOXM1 in human cancer cells.
The Journal of biological chemistry - 2 Dec 2011
Bhat Uppoor G, Jagadeeswaran Ramasamy, Halasi Marianna, Gartel Andrei L
Abstract excerpt
Using mass spectrometric analysis we found that oncogenic transcription factor FOXM1 that is overexpressed in a majority of human cancers interacts with multifunctional protein NPM, which is also overexpressed in a variety of human tumors. Coimmunoprecipitation and glutathione S-transferase pull-down experiments demonstrated that NPM forms a complex with FOXM1 and also identified the regions responsible for their...
Topics
- Animals
- Cell Line, Tumor
- Cell Nucleus
- Cytoplasm
- Forkhead Box Protein M1
- Forkhead Transcription Factors
- Gene Knockdown Techniques
- HEK293 Cells
- Humans
- Male
- Mice
- Mice, Nude
- Mutation
- Neoplasm Proteins
- Neoplasms
- Nuclear Proteins
- Nucleophosmin
- Protein Transport