Article
The telomeric Cdc13 protein interacts directly with the telomerase subunit Est1 to bring it to telomeric DNA ends in vitro.
Proceedings of the National Academy of Sciences of the United States of America - 20 Dec 2011
Wu Yun, Zakian Virginia A
Abstract excerpt
In Saccharomyces cerevisiae, a Cdc13-Est1 interaction is proposed to mediate recruitment of telomerase to DNA ends. Here we provide unique in vitro evidence for this model by demonstrating a direct interaction between purified Cdc13 and Est1. The Cdc13-Est1 interaction is specific and requires the in vivo defined Cdc13 recruitment domain. Moreover, in the absence of this interaction, Est1 is excluded from...
Topics
- Animals
- Cell Cycle
- DNA
- DNA, Single-Stranded
- Escherichia coli
- Kinetics
- Mutation
- Oligonucleotides
- Protein Binding
- Protein Structure, Tertiary
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Telomerase
- Telomere
- Telomere-Binding Proteins