Article
A Drosophila genetic screen yields allelic series of core microRNA biogenesis factors and reveals post-developmental roles for microRNAs.
RNA (New York, N.Y.) - 1 Nov 2011
Smibert Peter, Bejarano Fernando, Wang Dong, Garaulet Daniel L, Yang Jr-Shiuan, Martin Raquel, Bortolamiol-Becet Diane, Robine Nicolas, Hiesinger P Robin, Lai Eric C
Abstract excerpt
Canonical animal microRNAs (miRNAs) are ∼22-nt regulatory RNAs generated by stepwise cleavage of primary hairpin transcripts by the Drosha and Dicer RNase III enzymes. We performed a genetic screen using an miRNA-repressed reporter in the Drosophila eye and recovered the first reported alleles of fly drosha, an allelic series of its dsRBD partner pasha, and novel alleles of dicer-1. Analysis of drosha mutants...
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