Article
Herpes simplex virus 1 ICP0 phosphorylation site mutants are attenuated for viral replication and impaired for explant-induced reactivation.
Journal of virology - 1 Dec 2011
Mostafa Heba H, Thompson Thornton W, Kushnir Anna S, Haenchen Steve D, Bayless Adam M, Hilliard Joshua G, Link Malen A, Pitcher Lisa A, Loveday Emma, Schaffer Priscilla A, Davido David J
Abstract excerpt
In cell culture experiments, phosphorylation appears to be a critical regulator of the herpes simplex virus 1 (HSV-1) immediate-early (IE) protein, ICP0, which is an E3 ubiquitin ligase that transactivates viral gene expression. Three major regions of phosphorylation in ICP0 (amino acids 224 to 232, 365 to 371, and 508 to 518) have been identified, and mutant viruses that block phosphorylation sites within each...
Topics
- Amino Acid Sequence
- Animals
- Chlorocebus aethiops
- Eye Diseases
- Female
- Genome, Viral
- Herpes Simplex
- Herpesvirus 1, Human
- Immediate-Early Proteins
- Mice