Article
High resolution melting analysis for detection of BRAF exon 15 mutations in hairy cell leukaemia and other lymphoid malignancies.
British journal of haematology - 1 Dec 2011
Boyd Elaine M, Bench Anthony J, van 't Veer Mars B, Wright Penny, Bloxham David M, Follows George A, Scott Mike A
Abstract excerpt
The BRAF V600E mutation has recently been described in all cases of hairy cell leukaemia (HCL). We have developed and validated a rapid and sensitive high-resolution melting analysis (HRMA) assay that detects BRAF exon 15 mutations when hairy cells are as low as 5-10% in a sample. All 48 HCL patients were positive for the BRAF V600E mutation, while 114 non-HCL cases were all V600E negative. Interestingly, we...
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